21st century learning is required to be technology-based to balance the demands of the millennial era with the aim that students will become accustomed to 21st century life skills. The 21st century with the rapid development of information and technology also demands changes and adjustments in learning evaluation activities, one of which is based on computer-based tests ( CBT). This study aims to develop e-learning for General Chemistry courses on organic materials based on CBT and developed through the ADDIE model. This study produced a book and e-learning of General Chemistry on organic material based on a computer based test (CBT) and has been declared valid (appropriate) and proven effective to improve student HOTS achievement. The validity is met qualitatively based on the assessment of the expert validator and the effectiveness is met based on the implementation of CBT-based e-learning in organic learning as well as evidenced by the results of statistical hypothesis testing with a probability value of <0.05 with an average value of difference or increase in student HOTS of 40,167±11,099.