This research is motivated by a lack of learning resources about Biomedical II courses and learning media that are still not effective in improving learning outcomes. The purpose of this study was to produce a feasible and effective E-module in improving student learning outcomes in the Biomedical II course at Stikes Nurul Hasanah Kutacane. The development procedure used in this study is the development model from Borg and Gall and the instructional design step from Dick and Carey which is divided into 4 stages: the needs analysis stage, the product design stage, the validation and evaluation stage, and the final product stage. To see the effectiveness of the E-module, it can be analyzed through the normality test, homogeneity test, and hypothesis testing. Product validation results show a score percentage of 87% for media expert validation, 93% for material expert validation, and 96.18% for student response results. The results of the normality and homogeneity tests show that the research data has been declared normal and homogeneous. The results of the hypothesis test show that the value of t count is 3.03 and the value of t table is 1.66, where t count > t table. The results of this study indicate that the E-module is effective in improving student learning outcomes in Biomedical II at Stikes Nurul Hasanah Kutacane.