The implementation of global infection prevention policies necessitates a multi-faceted approach that integrates regulatory frameworks, ethical considerations, and biodefense strategies to mitigate the spread of infectious diseases. As emerging and re-emerging infections pose increasing public health threats, robust regulatory mechanisms are essential to ensure compliance with standardized infection control protocols. International health organizations, such as the World Health Organization (WHO) and the Centers for Disease Control and Prevention (CDC), play a pivotal role in establishing guidelines that govern disease surveillance, vaccine deployment, and antimicrobial resistance management. However, the effectiveness of these policies is often contingent on national regulatory frameworks, which must balance public safety, healthcare accessibility, and economic feasibility. Ethical considerations in infection prevention policies encompass the equitable distribution of medical resources, informed consent in public health interventions, and privacy concerns in disease surveillance technologies. Policymakers must navigate ethical dilemmas associated with quarantine measures, mandatory vaccination programs, and data-sharing protocols while maintaining public trust and cooperation. Furthermore, biodefense strategies, including pathogen threat assessment, biosecurity policies, and pandemic preparedness initiatives, are critical to strengthening global health security. The integration of artificial intelligence, genomic sequencing, and predictive modeling has enhanced early detection and response capabilities, yet gaps in policy coordination and funding allocation remain significant challenges. This paper examines case studies of past pandemics, evaluates the effectiveness of existing regulatory and ethical frameworks, and proposes a holistic approach to infection prevention policy implementation. By aligning regulatory measures with ethical imperatives and biodefense innovations, global health systems can achieve sustainable and resilient infection control strategies.